BEGIN:VCALENDAR VERSION:2.0 PRODID:icalendar-ruby CALSCALE:GREGORIAN X-WR-CALNAME:Physics Masters Thesis Defense – Zezhong Zhang\, Physics Gra duate Student\, “Relationship between Fibrin Clot Structure and Fibrinog en and Thrombin Concentrations” (Advisor: M. Guthold)) X-WR-TIMEZONE:Eastern Time (US & Canada) BEGIN:VEVENT DTSTAMP:20260810T163201Z UID:tag:localist.com\,2008:EventInstance_44932067250481 DTSTART:20231129T140000Z DTEND:20231129T150000Z DESCRIPTION:Abstract: \n\nFibrinogen is one of the most abundant proteins i n human blood plasma. In the event of an injury to the vasculature\, throm bin proteolytically cleaves two pairs of fibrinopeptides off fibrinogen\, and thereby converts it to fibrin. Fibrin polymerizes into fibrin fibers\, which form a mesh that stabilizes a blood clot. This process plays an imp ortant role in thrombosis and hemostasis. In this research\, we explored t he relationship between structural properties (fiber density\, pore size a nd fractal dimension) of a fibrin clot and concentrations of fibrinogen an d thrombin. We also explored the effect of fibrinogen types - purified fib rinogen and fibrinogen in plasma - on fibrin structural properties. Confoc al microscopy is the imaging technique we used in this research. We discov ered that increasing fibrinogen concentrations\, and increasing thrombin c oncentrations resulted in clots with higher fiber density\, smaller pore s ize and higher fractal dimension. Clots formed from purified PEAK1 fibrino gen\, instead of plasma fibrinogen\, also showed higher fiber density\, sm aller pore size and higher fractal dimension. Based on these results\, we found power law equations that relate structural properties to fibrinogen and thrombin concentrations. The equations indicate that fibrinogen concen tration has a stronger effect on clot structure than thrombin concentratio n. We discuss implications of these results for clot formation\, future re search directions\, and the importance of our results to thrombosis and he mostasis.Fibrinogen is one of the most abundant proteins in human blood pl asma. In the event of an injury to the vasculature\, thrombin proteolytica lly cleaves two pairs of fibrinopeptides off fibrinogen\, and thereby conv erts it to fibrin. Fibrin polymerizes into fibrin fibers\, which form a me sh that stabilizes a blood clot. This process plays an important role in t hrombosis and hemostasis. In this research\, we explored the relationship between structural properties (fiber density\, pore size and fractal dimen sion) of a fibrin clot and concentrations of fibrinogen and thrombin. We a lso explored the effect of fibrinogen types - purified fibrinogen and fibr inogen in plasma - on fibrin structural properties. Confocal microscopy is the imaging technique we used in this research. We discovered that increa sing fibrinogen concentrations\, and increasing thrombin concentrations re sulted in clots with higher fiber density\, smaller pore size and higher f ractal dimension. Clots formed from purified PEAK1 fibrinogen\, instead of plasma fibrinogen\, also showed higher fiber density\, smaller pore size and higher fractal dimension. Based on these results\, we found power law equations that relate structural properties to fibrinogen and thrombin con centrations. The equations indicate that fibrinogen concentration has a st ronger effect on clot structure than thrombin concentration. We discuss im plications of these results for clot formation\, future research direction s\, and the importance of our results to thrombosis and hemostasis.Fibrino gen is one of the most abundant proteins in human blood plasma. In the eve nt of an injury to the vasculature\, thrombin proteolytically cleaves two pairs of fibrinopeptides off fibrinogen\, and thereby converts it to fibri n. Fibrin polymerizes into fibrin fibers\, which form a mesh that stabiliz es a blood clot. This process plays an important role in thrombosis and he mostasis. In this research\, we explored the relationship between structur al properties (fiber density\, pore size and fractal dimension) of a fibri n clot and concentrations of fibrinogen and thrombin. We also explored the effect of fibrinogen types - purified fibrinogen and fibrinogen in plasma - on fibrin structural properties. Confocal microscopy is the imaging tec hnique we used in this research. We discovered that increasing fibrinogen concentrations\, and increasing thrombin concentrations resulted in clots with higher fiber density\, smaller pore size and higher fractal dimension . Clots formed from purified PEAK1 fibrinogen\, instead of plasma fibrinog en\, also showed higher fiber density\, smaller pore size and higher fract al dimension. Based on these results\, we found power law equations that r elate structural properties to fibrinogen and thrombin concentrations. The equations indicate that fibrinogen concentration has a stronger effect on clot structure than thrombin concentration. We discuss implications of th ese results for clot formation\, future research directions\, and the impo rtance of our results to thrombosis and hemostasis.\n\n \n\nSee Flyer GEO:36.1321;-80.27898 LOCATION:Olin Physical Laboratory\, 107 SUMMARY:Physics Masters Thesis Defense – Zezhong Zhang\, Physics Graduate Student\, “Relationship between Fibrin Clot Structure and Fibrinogen an d Thrombin Concentrations” (Advisor: M. Guthold)) URL;VALUE=URI:https://events.wfu.edu/event/physics_masters_thesis_defense_z ezhong_zhang_physics_graduate_student_relationship_between_fibrin_clot_str ucture_and_fibrinogen_and_thrombin_concentrations_advisor_m_guthold CATEGORIES:Special Events END:VEVENT END:VCALENDAR